What is the step-by-step process of respiration?
Respiration is a multi-stage biochemical process in which glucose is broken down to release energy in the form of ATP. It occurs in three main stages: glycolysis, Krebs cycle, and electron transport chain.
Step-by-step process of respiration:
1. Glycolysis (in cytoplasm)
Glucose (6-carbon) is split into two molecules of pyruvate (3-carbon each).
A small amount of energy (ATP) and NADH is produced.
Does not require oxygen (can occur in both aerobic and anaerobic conditions).
2. Link reaction (transition step)
Pyruvate enters mitochondria and is converted into acetyl-CoA.
Carbon dioxide is released and NADH is formed.
3. Krebs cycle (Citric Acid Cycle – in mitochondria)
Acetyl-CoA is completely broken down.
Produces:
Carbon dioxide (CO₂)
NADH and FADH₂ (electron carriers)
A small amount of ATP
4. Electron Transport Chain (ETC – inner mitochondrial membrane)
NADH and FADH₂ donate electrons.
Energy released is used to produce a large amount of ATP.
Oxygen acts as the final electron acceptor and forms water.
Total outcome (aerobic respiration):
High ATP production
CO₂ and H₂O as by-products
In anaerobic conditions:
Only glycolysis occurs
Pyruvate is converted into lactic acid (in muscles) or alcohol (in yeast)
Much less ATP is produced
In simple terms: Respiration breaks glucose step by step—first in cytoplasm, then in mitochondria—to release energy stored as ATP.